要: 【目的】通过理论预测与试验验证, 旨在揭示miR-433-3p对BCKDHB的调节机制。【方法】利用Target Scan、miRanda和DIANA-micro T 3个在线软件, 以BCKDHB的序列预测与BCKDHB有靶标关系的相关miRNAs。为了验证理论上的预测结果, 用设计好的BCKDHB 3′-UTR的特异性引物进行PCR扩增, 得到目的片段并进行割胶回收和纯化,...
Pathway- Gene SummaryThis gene encodes the beta chain of the branched chain alpha ketoacid dehydrogenase (Bckdh) complex. The encoded protein exists in a heterotetrameric complex containing the Bckdh alpha subunit to form the E1 catalytic component of Bckdh complex. The Bckdh complex catalyzes th...
Pathway- Gene SummaryThis gene encodes the E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in this gene have been ass...