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The primers used amplified a partial sequence of the VP1 protein, spanning from 1630 bp to 1956 bp in sequence NC_001538 from the NCBI database [47]. The reaction was carried out with primers 327-1PST (5′ CAAGTGCCAAAACTACTAAT 3′) and 327-2HIN (5′ GCATGAAGGTTAAGCATGC 3′) ...