As it is now possible to crystallize chymotrypsinogen salt free from aqueous alcohol 5 , it was thought worth while to attempt to determine the isoelectric point of such a preparation by a method based on the Donnan equilibrium.doi:10.1038/170250a0INGRAM...
doi:10.1016/S0021-9258(18)93279-5J C NicholJournal of Biological ChemistryNichol, L. W. (1968). "Sedimentation behavior of chymotrypsinogen A in the vicinity of the isoelectric point." J. Biol. Chem. 243: 4065-4069.
The progressive transition of CTG-A () to CTG-X (), a previously described biologically active carbamylated derivative (1–3), is demonstrated by isoelectric focusing in polyacrylamide gels. This technique allowed the visualization of 12 carbamylated protein species, whose isoelectric point could be...
A homogeneous preparation of chymotrypsinogen has been obtained by the fractional salting out of an acid extract of porcine pancreas and subsequent chromatography on a column of CM-cellulose. The isoelectric point of the enzyme has been found to be pH 8.8 and the optimum value of the pH for ...
a prediction could be made of the conditions under which the carbamylated products would exhibit maximum solubility.These results demonstrate the high resolving power and the potential of gel isoelectric focusing for following the course and determining the end-point of reactions of proteins in which ...
p I, isoelectric pointAbsorption spectra of aqueous solutions of Rose Bengal—(α-chymotrypsin) and Rose Bengal—(α- chymotrypsinogen-A) measured at different pHs showed that both proteins formed the same complexes. The binding constants for the formation of RB/CHT and RB/CHTGA complexes were ...
Sea bream CHTRII appears to be the longest of all known teleostean chymotrypsinogen forms and contains a high number of methionine residues. Compared with CHTRI, CHTRII is more hydrophobic and has a lower isoelectric point. On the other hand, deduced amino acid sequence of TRPII is 241 aa ...
Trypsinogen 1 and chymotrypsinogen can only be separated at this pH, which is close to their isoelectric point. Each of the three purified proenzymes shows a single band on polyacrylamide gel disc electrophoresis. 4. 4.|The amino acid composition of rat chymotrypsinogen resembles those of beef ...