72 Klement JF, Rice NR, Car BD, Abbondanzo SJ, Powers GD, Bhatt PH, Chen CH, Rosen CA, Stewart CL. IkappaBalpha de- ficiency results in a sustained NF-kappaB response and severe widespread dermatitis in mice. Mol Cell Biol 1996; 16:2341- 2349. 73 Rebholz B, Haase I, Eckelt B,...
Matrigel assay with HUVECs was performed as previously described using BD Matrigel Basement Membrane Matrix (BD Biosciences)56. RNA extraction and quantitative real-time analysis Total RNA was extracted using the miReasy kit (Qiagen). Real-time quantification to measure miRNAs was performed with the...
Hypoxia and inflammation are coincidental events in an array of diseased tissues, including chronically inflamed sites (e.g., inflammatory bowel disease, rheumatoid arthritis), growing tumors, myocardial infarcts, atherosclerotic plaques, healing wounds, and sites of bacterial infection (Murdoch et al. ...
Mtb genome encodes for 23 (EsxA-EsxW) proteins belonging to the ESAT-6 like family; however, most of them are functionally unknown. Here, we show that Mtb EsxL induces tumor necrosis factor-alpha (TNF-α) production by activating nuclear translocation of nuclear factor-κB (NF-κB) via ...
Possible role of NF-kappaB and p53 in the glutamate-induced pro-apoptotic neuronal pathway. Cell death and differentiation is a monthly research journal focused on the exciting field of programmed cell death and apoptosis. It provides a single acc... M Grilli,M Memo - 《Cell Death & ...
Transforming growth factor (TGF)-β is the prototype of a large superfamily of signaling molecules involved in the inhibition of proliferation of multiple epithelial cell types. Although accumulated evidence indicates the mechanisms of the antimitogenic effect of TGF-β in a variety of cell types, ...
The transcription factor NF-κB has been shown to be predominantly cytoplasmically localized in the absence of an inductive signal. Stimulation of cells with inflammatory cytokines such as tumor necrosis factor α or interleukin-1 induces the degradation of IκB, the inhibitor of NF-κB, allowing ...
The fixed cells were stained in PBS containing 100 μg/ml propidium iodide (P-4170, Sigma) and 20 μg/ml RNase A (catalog No. 12091-039, Invitrogen) followed by analysis in BD LSRFortessa using BD FACSDiva 6.2 software. FITC–annexin V staining for cell death determination Apoptosis in ...
Samples containing equal amounts of protein were resolved by SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% Difco skimmed milk (BD, Shanghai, China) and then incubated with primary and secondary antibodies. The antibodies used in...
Anti-CD31 antibody was obtained from BD Pharmingen (San Diego, CA). Reactive oxygen species detection reagents were obtained from Invitrogen Molecular Probes (Eugene, OR). MTT assay kit was purchased from Roche Molecular Biochemicals (Indianapolis, IN). Matrigel Invasion Chambers were purchased from ...