Method for transient expression of (poly)peptides (I) in mammalian cells (A). Method for transient expression of (poly)peptides (I) in mammalian cells (A) comprises: (a) growing (A) in a 70:30-30:70 mixture (M) of calcium-free, vitamin- and amino acid-supplemented Dulbecco's ...
DEAE-dextran, a cationic polymer, was one of the first chemical reagents used to transfect cultured mammalian cells (Vaheri and Pagano, 1965; McCutchan and Pagano, 1968). An excess of positive charge, contributed by the polymer in the DNA:polymer complex, allows the complex to come into cl...
the applications section of this video-article describes the use of electroporation and a biolistic transfection as alternative methods for introducing nucleic acid into mammalian cells. It also describes an advanced use of transfection where co-transfection of interfering RNA and DNA are introduced as ...
doi:10.1038/nmeth0106-67NoneNature MethodsTransfection of mammalian cells by electroporation, Nature Methods 3 (2006) 67-68.Transfection of mammalian cells by electroporation. Nat Meth 2006, 3(1):67- 68.
Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies no...
Recently developed transfection methods for mammalian cells provide a powerful means for the study of gene function. Unfortunately, human endothelial cells were relative refractory to the classic transfection techniques. In this study we compared the usability of calcium phosphate, DEAE-dextran transfection...
METHODS OF TREATING KIDNEY DISEASE In one aspect, the invention features methods of treating, or reducing risk...In mammalian cells, RNAi can be triggered by 21-nucleotide (nt) duplexes ...known in the art, e.g., cationic liposome transfection and electroporation... A Greka - US 被引量...
a method of introducing the purifiedDNAof aVIRUSintoCELLS. Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005 trans·fec·tion (trans-fek'shŭn) A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) ...
A novel transfection method for mammalian cells using gas plasma. Sakai Y,Khajoee V,Ogawa Y,et al. Journal of Biotechnology . 2006Sakai Y,Khajoee V,Ogawa Y,et al.A novel transfection method for mammalian cells using gas plasma.Journal of Biotechnology. 2006...
proteinintomammaliancells.Undernormalconditions,mammaliancellstakeupandexpressexternallyappliedDNAwithverylowe ciency. isismostlyduetothelipidbilayeroftheeukaryoticcellmembrane,whichactsasabarriertotheentryofchargedmoleculesintoacell.Severaltransfectionmethodshavebeendevelopedtoovercomethisproblem.Withthesemethods,thestudyof...