Transfection of mammalian cells. Alyson L. Peel. Methods . 2004A. Peel, " Transfection of Mammalian Cells ", Methods, vol. 33 (2004) pp. 93-94.Peel, A., ed. Transfection of mammalian cells, in Methods , vol. 33, No 1, 2004....
The optimal conditions for electroporation of untested cell lines must be determined experimentally.doi:10.1038/nmeth0106-67NoneNature MethodsTransfection of mammalian cells by electroporation, Nature Methods 3 (2006) 67-68.Transfection of mammalian cells by electroporation. Nat Meth 2006, 3(1):67- ...
Here, we provide a first, fundamental step towards biological reactions in air by demonstrating the acoustophoretic DNA transfection of mammalian cells. We developed an original acoustophoretic design capable of levitating, moving and mixing biological suspensions of living mammalians cells and of DNA ...
DEAE-dextran, a cationic polymer, was one of the first chemical reagents used to transfect cultured mammalian cells (Vaheri and Pagano, 1965; McCutchan and Pagano, 1968). An excess of positive charge, contributed by the polymer in the DNA:polymer complex, allows the complex to come into cl...
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Cells with different biological features require varying transfection conditions to obtain higher transfection efficiency of target genes. 展开 关键词: Electroporation transfection Mammalian cell Parameter optimization DOI: 10.1007/s00232-012-9480-0 被引量: 27 ...
Histones, known as natural DNA-binding proteins, have been utilized to condense nucleic acids and achieve the gene transfection in mammalian cells (Haberland and Bottger, 2005). In eukaryotes, histones are the main components of chromatin, and also octamers of small, basic proteins with high cont...
mouse embryonic stem cellsLiterature reports graphene, an atomic-thick sheet of carbon atoms as one of the promising biocompatible scaffolds that promotes cellular proliferation in human mesenchymal stem cells. On the other hand, different mammalian cell lines including the induced pluripotent ste...
Recently developed transfection methods for mammalian cells provide a powerful means for the study of gene function. Unfortunately, human endothelial cells were relative refractory to the classic transfection techniques. In this study we compared the usability of calcium phosphate, DEAE-dextran transfection...
Splicing promotes rapid and efficient mRNA export in mammalian cells The numerous steps in protein gene expression are extensively coupled to one another through complex networks of physical and functional interactions. Inde... P Valencia,AP Dias,R Reed - 《Proceedings of the National Academy of ...